mouse neuroblastoma neuro2a cells  (ATCC)

Journal: bioRxiv

Article Title: A Retinoic Acid Autoregulatory Loop Governing Prefrontal-Motor Arealization

doi: 10.64898/2025.12.23.696038

Figure Lengend Snippet: a, Whole-mount b-Galactosidase staining of the RARE – lacZ mouse brains at PCD 13 and 16. b, Meis2 promoter luciferase assay in Neuro2a cells with mouse Rxrg and Rarb . c, Meis2 expression in PD 0 Rarb/Rxrg double-knockout (dKO) and control mice, showing reduced Meis2 expression in the mPFC. Enlarged views (c′–c′′) highlight Meis2 ISH signal in the mPFC. Scale bars, see images; statistical details, see Methods.

Article Snippet: Neuro2a mouse neuroblastoma cell line was purchased from ATCC.

Techniques: Staining, Luciferase, Expressing, Double Knockout, Control

Journal: bioRxiv

Article Title: A Retinoic Acid Autoregulatory Loop Governing Prefrontal-Motor Arealization

doi: 10.64898/2025.12.23.696038

Figure Lengend Snippet: a, CUT&Tag profiles of H3K4me3, H3K27ac and MEIS2 binding in the human mid-fetal dlPFC. Genome-browser views show representative cortical layer genes ( SATB2 , BCL11B ). b, CUT&Tag profiles of H3K4me3, H3K27ac and MEIS2 binding in the human mid-fetal dlPFC. Genome-browser views show MEIS2 binding peaks at the MEIS2 locus. c, Mouse Meis2 promoter luciferase assay in Neuro2a cells with increasing concentrations of mouse MEIS2. Statistical details, see Methods.

Article Snippet: Neuro2a mouse neuroblastoma cell line was purchased from ATCC.

Techniques: Binding Assay, Luciferase

Journal: Biology Open

Article Title: ATP increases murine neuroblastoma cell size through a PANX1- and macropinocytosis-dependent mechanism

doi: 10.1242/bio.062272

Figure Lengend Snippet: Elevated extracellular ATP increases N2a cell size through a putative PANX1- and macropinocytosis-dependent mechanism. (A) Representative confocal micrographs of PANX1-EGFP (green) N2a cells overlaid with brightfield imaging following incubation with vehicle (water) or ATP (500 μM; 30 min). (B) Quantification of cross-sectional cellular area in PANX1-EGFP (representative outlined traces) or PANX1 W74A-EGFP N2a cells following treatment with vehicle (water) or ATP (500 μM). One-way ANOVA with Dunnett's post hoc ( N =3). (C) Representative confocal micrographs of PANX1-EGFP (green) N2a cells pre-treated with vehicle (DMSO), or amiloride (300 μM) for 1 h prior to vehicle (water) or ATP (500 μM; 30 min). (D,E) Cross-sectional cellular area (D) and intracellular PANX1 (E) was quantified relative to vehicle control. N =4, two-way ANOVA with Dunnett's post hoc [interaction: F (1,12)=19.53, P =0.0008; inhibitor: F (1,12)=5.825, P =0.0327; ATP: F (1,12)=22.22, P =0.0005]. Hoechst (cyan) was used as a nuclear counterstain. (F) Representative confocal micrographs of CHX-treated PANX1-RFP (green) N2a cells transiently transfected with ARF6-CFP or ARF6-Q67L-CFP (magenta, 48 h) prior to vehicle (water) or ATP (500 μM; 30 min), indicating accumulation of intracellular PANX1 with co-expression of constitutively active ARF6 mutant. White arrowheads indicate overlap of internalized vesicles. (G,H) Cross-sectional cellular area (G) and intracellular PANX1 (H) was quantified for each ARF6 construct relative to vehicle treatment. N =3, (unpaired t -tests, WT: P =0.026, Q67L: P =0.96). (I) Representative confocal micrographs demonstrating accumulation of PANX1RFP (green) and ARF6-Q67L-CFP (magenta) in early endosomes (EEA1, cyan). N refers to the number of coverslips from independent passages (≥30 cells analysed per coverslip). Individual data points are represented with mean±s.e.m. * P <0.05, ** P <0.01, **** P <0.0001. Scale bars: 10 μm.

Article Snippet: Neuro2a (N2a) mouse neuroblastoma cells (procured from the American Type Culture Collection) were cultured in Dulbecco's modified Eagle's medium (DMEM)/F12 supplemented with 10% FBS, 100 units/ml penicillin, and 100 mg/ml streptomycin (all obtained from Gibco/Life Technologies) and routinely assessed for contamination.

Techniques: Imaging, Incubation, Control, Transfection, Expressing, Mutagenesis, Construct

Journal: Biology Open

Article Title: ATP increases murine neuroblastoma cell size through a PANX1- and macropinocytosis-dependent mechanism

doi: 10.1242/bio.062272

Figure Lengend Snippet: Intracellular PANX1 distributes to macropinosomes. (A) Representative confocal micrographs with line scan over ROI demonstrate PANX1 localization in membrane-like structures surrounding macropinosome cargo 70 kDa dextran-TRITC. Inset highlights putative PANX1-positive macropinosome. (B) Line scan (10 µm) of putative macropinosome with PANX1-encompassing dextran-TRITC cargo. (C) Diameter of intracellular PANX1, TRITC-Dextran, and co-distributed structures show similar size distribution pattern. (D) Representative confocal micrographs of transiently expressed PANX1-EGFP (green) N2a cells pre-treated with vehicle or EIPA (25 μM), LY-294002 (25 µM), or latrunculin-A (15 µM) then incubated with 70 kDa-dextran-TRITC (100 µg/ml, magenta) and ATP (500 μM, 30 min) prior to fixation. (E) Intracellular PANX1 following ATP stimulation (30 min) was quantified relative to vehicle control. N =14-15 per treatment group, one-way ANOVA with Dunnett's post hoc [interaction: F (3,55)=9.124, P <0.0001]. (F) Co-distribution rate of intracellular PANX1 with TRITC-dextran following ATP stimulation for each treatment ( N =5 per treatment group, one-way ANOVA with Dunnett's post hoc [interaction: F (3,16)=164, P <0.0001]. * P <0.05, ** P 0.01, **** P <0.0001. N refers to the number of coverslips from independent passages (≥30 cells analyzed per coverslip). Individual data points are represented with mean±s.e.m. Scale bars: 10 μm.

Article Snippet: Neuro2a (N2a) mouse neuroblastoma cells (procured from the American Type Culture Collection) were cultured in Dulbecco's modified Eagle's medium (DMEM)/F12 supplemented with 10% FBS, 100 units/ml penicillin, and 100 mg/ml streptomycin (all obtained from Gibco/Life Technologies) and routinely assessed for contamination.

Techniques: Membrane, Incubation, Control